癸培养基的研制及在小麦花药培养中的应用研究

在多年的小麦(Triticum aestivum L.)花药培养育种及对多种培养基应用研究的基础上,从调整培养基的大量元素和微量元素入手,建立了癸培养基。经过L_(16)(4~5)正交实验,筛选出NH_4N0_3、KNQ_3、MgSO_4、MnSO_4在培养基中较优的配比。在培养基对比实验中,癸培养基的愈伤组织平均诱导率分别比C_(17)、W_(14)、N_6培养基提高了30.31％、50.60％和57.96％,且对不同的杂交组合都能表现出优越性。在癸培养基中附加0.9mg/L REA(rare eaxth addition,稀土元素附加剂),可使小麦花药愈伤组织诱导率提高54.25％～64.07％,并对愈伤组织的生长有促进作用。

PREPARATION OF GUI CULTURE MEDIUM AND ITS APPLICATION TO WHEAT ANTHER CULTURE

Gui culture medium has been developed by adjusting the concentration of macro- and micro- elements, based on the studies of various media applying to wheat ( Triticum aestivum L.) anther culture breeding for many years. An appropriate ration among NH4NO3, KNO3, MgSO4 and MnSO4 in the medium was selected by orthogonal design L16(45). For comparison of different media namely C17, W14 and N6 medium, the average frequencies of callus induction were increased by 30.31%, 50.66% and 57.96% respectively when Gui medium was used. And the Gui medium showed all its advantages in various crosses. The frequencies of wheat anther callus induction showed an increase of 54.25% to 64.07% and the callus development was also promoted when adding 0.9 mg/L rare earth addition to the Gui medium.