林生山黧豆中2,4—二氨基丁酸合成代谢的体外酶学研究 |
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引用本文: | 穆小民,沈透明.林生山黧豆中2,4—二氨基丁酸合成代谢的体外酶学研究[J].Acta Botanica Sinica,1997,39(6):537-540. |
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作者姓名: | 穆小民 沈透明 |
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作者单位: | 中国农业大学生物学院,中国农业大学生物学院,中国农业大学生物学院 北京 100994 中国科学院动物研究所生殖生物学国家重点实验室,北京100080,北京 100994,北京 100994 |
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摘 要: | 用~3H-天门冬氨酸为底物,林生山黧豆(Lathyrus sylvestris L.)叶片匀浆上清液为粗酶液,进行体外反应。结果表明,天门冬氨酸的放射性掺入到2,4-二氨基丁酸,加入谷氨酸则能抑制这种掺入。将上述粗酶液透析,加入可能的辅助因子,天门冬氨酸的放射性也掺入到2,4-二氨基丁酸。研究证实在体外天门冬氨酸可以作为2,4-二基丁酸合成的底物,在林生山黧豆体内存在催化天门冬氨酸转变为2,4-二氨基丁酸的合成酶(系)。以2,4-二氨基丁酸和γ-氨基丁酸为底物,用氨基酸自动分析仪测定产物含量,结果表明,2,4-二氨基丁酸和γ-氨基丁酸不互相转变。
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关 键 词: | 2 4-二氨基丁酸 合成代谢 3~H-天门冬氨酸 γ-氨基丁酸 林生山黧豆 |
IN VITRO ENZYMOLOGICAL STUDIES ON ANABOLISM OF 2,4-DIAMBVOBUTYRIC ACID IN LATHYRUS SYLVESTRIS |
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Authors: | Mu Xiao-min Shen Li-ming and Wu Xian-rong |
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Abstract: | An in vitro synthetic reaction system was established with 2,3-3H-aspartic acid (Asp) as a substrate and the homogenate of flatpea (Lathyrus sylvestris L.) leaves as the crude enzyme extract. The results showed that 3H-Asp was incorporated into 2,4-diaminobutyric acid (DABA). The incorporation was inhibited by the addition of glutamic acid (Glu). 3H-Asp was also incorporated into DABA after the crude enzyme was dialyzed, indicating that Asp as a substrate for DABA synthesis was catalyzed by a group of enzymes which converted Asp to DABA in flatpea. From the in vitro reactions it was proved that DABA and y-aminobutyric acid (GABA) could not be mutually substituted as substrates. |
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Keywords: | 2 4-diaminobutyric acid Anabolism 3H-aspartic acid r-aminobutyric acid Lath-yrus sylvestris |
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