毛花猕猴桃原生质体再生植株根尖染色体数目变异与细胞多核现象 |
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引用本文: | 张远记,魏小萍.毛花猕猴桃原生质体再生植株根尖染色体数目变异与细胞多核现象[J].Acta Botanica Sinica,1997,39(2):102-105. |
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作者姓名: | 张远记 魏小萍 |
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作者单位: | 中国科学院植物研究所,中国科学院植物研究所,中国科学院植物研究所,中国科学院植物研究所,北京市植物细胞工程实验室,中国科学院植物研究所 北京 100093,北京 100093,北京 100093,北京 100093,北京 100081,北京 100093 |
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摘 要: | 对18株毛花猕猴桃(Actinidia eriantha Benth.)原生质体再生植株的体细胞染色体数做了观察,其中12株为整倍体:二倍体(2n=58)和四倍体(2n=116)各6株;另外6株为混倍体,其染色体数目变化在59~203之间。还发现原生质体再生植株有丝分裂间期细胞存在多核现象,有多核细胞的共10株,细胞核数目以双核和三核较常见,最多的有7个核。对照植株为2n=2x=58,未发现多核细胞。
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关 键 词: | 毛花猕猴桃 原生质体再生植株 染色体数目 多核现象 |
SOMACLONAL VARIATION IN CHROMOSOME NUMBER AND NUCLEI NUMBER OF REGENERATED PLANTS FROM PROTOPLASTS OF ACTIN1DIA ERIANTHA |
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Authors: | Zhang Yuan-ji Qian Ying-qian Cai Qi-gui Mu Xi-jin Wei Xiao-ping and Zhou Yun-luo |
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Abstract: | By counting the chromosome number of root tip cells in 18 regenerated plants derived from protoplasts of Actinidia eriantha Benth. ,the authors found 12 euploid plants and 6 mixoploid plants. Of the 12 euploid plants,6 were diploid (2n = 2x = 58) and the other 6 were tetraploid (2n = 4x = 116). The chromosome numbers of the mixoploid plants varied from 59 to 203. Multinucle-ate phenomenon was also observed in the interphase cells of 10 protoplast-derived plants. Cells with binuclei or trinuclei were common and cells having heptanuclei were also seen occassionally. Multin-ucleate phenomenon did not occur in the control, i. e. , the donor plant, where the chromosome number was 2n = 2x = 58. |
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Keywords: | Actinidia eriantha Protoplast-derived plants Chromosome number Multinucleate phenomenon |
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