| 被孢霉菌发酵产生花生四烯酸的研究 |
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| 引用本文： | 鲍时翔,朱法科,林炜铁,姚汝华.被孢霉菌发酵产生花生四烯酸的研究[J].微生物学报,1997,37(5):374-377. |
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| 作者姓名： | 鲍时翔 朱法科 林炜铁 姚汝华 |
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| 作者单位： | 华南理工大学生物工程系；华南理工大学生物工程系 广州 |
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| 基金项目： | 中国博士后科学基金，广东省自然科学基金资助项目 |
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| 摘 要： | 研究了温度、培养基初始pH、碳源、氮源对被孢霉(Mortierella sp.)产生花生四烯酸的影响。正交试验结果表明,Mortierella sp.M10最佳培养基组成为(g/L):葡萄糖100,酵母膏10,KNO_3 4.0,KH_2PO_4 2.0,CaCl_2·2H_2O 0.1,MgSO_4·7H_2O 0.5,FeCl_3·6H_2O0.015,ZnSO_4·7H_2O 0.0075,CuSO_4·5H_2O 0.0005。采用最佳培养基及发酵条件,细胞干重和花生四烯酸产量分别为33.51g/L和0.827g/L。同时对摇瓶发酵过程进行了分析。
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| 关 键 词： | 花生四烯酸 发酵 被孢霉 |
STUDIES ON ARACHIDONIC ACID PRODUCTION BY MORTIERELLA |
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| Bao Shixiang Zhu Fake Lin Weitie Yao Ruhua.STUDIES ON ARACHIDONIC ACID PRODUCTION BY MORTIERELLA[J].Acta Microbiologica Sinica,1997,37(5):374-377. |
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| Authors: | Bao Shixiang Zhu Fake Lin Weitie Yao Ruhua |
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| Institution: | Dept. of Bioengineering, South China University of Technology, Guangzhou 510641. |
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| Abstract: | The effects of the incubation temperature, initial pH of the medium, carbon source and nitrogen source on the production of arachidonic acid by Mortierella sp. M10 were studied. Thought orthogonal experiments, the optimum culture medium was obtained (g/L): glucose, 100; yeast extract, 10; KNO3, 4.0; KH2PO4, 2.0; CaCl2.2H2O, 0.1; MgSO4.7H2O, 0.5; FeCl3.6H2O, 0.015; ZnSO4.7H2O, 0.0075; CuSO4.5H2O, 0.0005. Under the optimum culture conditions, the dry cell weight and arachidonic acid was 33.51 g/L and 0.827 g/L, respectively. The flask culture process was analysed. |
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| Keywords: | Arachidonic acid Fermentation Mortierella sp |
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