蓝光对绿豆下胚轴愈伤组织生长和呼吸的影响

与白光和黑暗条件相比,蓝光明显促进绿豆(Vigna radiata (Linn.)Wilczek)下胚轴愈伤组织的形成和生长。培养到18d,蓝光下生长的愈伤组织鲜重分别是白光和黑暗下的120％和191％。三种条件下愈伤组织的呼吸速率有明显的不同,培养5d,呼吸速率以蓝光处理的最高,白光处理的次之,黑暗处理的最低;培养14d,与上述相反,呼吸速率由高到低则依次是黑暗、白光和蓝光。应用呼吸抑制剂碘乙酸、丙二酸和正磷酸钠试验及对6-磷酸-葡萄糖脱氢酶活性测定的结果表明,培养5d,蓝光和白光处理时磷酸已糖支路(HMP)明显增加,被碘乙酸 丙二酸抑制后的呼吸占总呼吸的34％～36％,黑暗处理的仅占总呼吸9％～11％ 绿豆下胚轴愈伤组织中亦存在抗氰交替途径,黑暗处理时,此种途径的最大容量明显高于蓝光和白光处理的,培养14d,黑暗处理占总呼吸的31％左右.而蓝光和白光处理的则占20％以下。

EFFECTS OF BLUE LIGHT ON CALLUS GROWTH AND RESPIRATION IN HYPOCOTYL OF MUNG BEAN

The effects of blue light (BL) on callus growth and respiration in hypocotyl callus of mung bean ( Vigna radiata (Linn. ) Wilczek) were investigated. The results showed that BL could promote callus formation and growth as compared with white light (WL) and the darkness (D). On the eighteenth day after culture, the fresh weight of calli cultured in BL was 120% and 191.5% of those in WL and in D respectively. The respiratory rate in calli measured on the fifth day after culture was greatest in BL. and lowest in D. However, the order of respiration rate was reversed when measured on the fourteenth day after culture. The results from the experiments with inhibitors (iodoacetic acid malonate, or Na3PO4) and by measuring the activity of phosphogloconic dehydro-genase(G-6-PDH) suggested that hexose monophospate pathway (HMP) increased in BL and in WL 5 days later, because the residual respiration inhibited by iodocacetic acid malonate was 34% -36% of total one, which was inhibited by Na3PO4; While it was 9% - 11% in D. G-6-PDH activity in BL was much greater than in D, especially the fifth day after culture. The cyanide-resistant pathway occurred in mung bean hypocotyl. The capacity of this respiration in D was greater than that in BL or in WL. The fourteenth day after culture, it reached about 31% in D, but less than 20% in BL or in WL.