cDNA encoding a wheat (Triticum aestivum cv. Chinese Spring) glycine-rich RNA-binding protein |
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Authors: | Mark J. Guiltinan Xiping Niu |
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Affiliation: | (1) Department of Horticulture, The Biotechnology Institute, Intercollege Program in Plant Physiology, The Center for Gene Regulation, The Pennsylvania State University, 519 Wartik Laboratory, 16802 University Park, PA, USA |
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Abstract: | A wheat cDNA encoding a glycine-rich RNA-binding protein, whGRP-1, was isolated. WhGRP-1 contains two conserved domains, the RNA-binding motif (RNP motif) combined with a series of glycine-rich imperfect repeats, characteristic of a conserved family of plant RNA-binding proteins. Northern analysis revealed that whGRP-1 mRNA accumulates to high levels in roots and to lower levels in leaves of wheat seedlings. whGRP-1 mRNA accumulation is not enhanced by exogenous abscisic acid in seedlings and accumulates to very high levels during wheat embryo development, showing a pattern different from that of the ABA-inducible wheat Em gene. |
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Keywords: | abscisic acid glycine-rich ribonucleoprotein RNA-binding protein RNP motif wheat |
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