Crystallographic characterization of a principal non-toxic lectin from seeds of Ricinus communis |
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Authors: | Chin Hsuan Wei Chongkun Koh |
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Institution: | Biology Division Oak Ridge National Laboratory Oak Ridge, Tennessee 37830, U.S.A. |
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Abstract: | Large hexagonal crystals of ricinus lectin, present as a major component in the seeds of Ricinus communis, have been obtained at 4 °C in the presence of polyethylene glycol 6000 by vapor diffusion against media containing acetic acid. The crystals are of space group P622, with hexagonal unit cell parameters . The asymmetric unit contains two molecules of molecular weight 125,000. The crystals are extremely unstable, both to environmental changes and to X-irradiation, and have a solvent content of approximately 55% by volume. |
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