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Saponin production is not qualitatively changed upon callus regeneration in the medicinal shrub Maesa perlarius |
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| Authors: | Ahmad Faizal Kenn Foubert Ellen Lambert Nico De Storme Magda Claeys Sandra Apers Danny Geelen |
| Affiliation: | 1. Department of Plant Production, Faculty of Bioscience Engineering, Ghent University, Coupure Links 653, 9000, Ghent, Belgium 2. Laboratory of Pharmacognosy and Pharmaceutical Analysis, Department of Pharmaceutical Sciences, Faculty of Pharmaceutical, Biomedical and Veterinary Sciences, University of Antwerp, Universiteitsplein 1, 2610, Antwerp, Belgium 3. Laboratory of Mass Spectrometry, Department of Pharmaceutical Sciences, Faculty of Pharmaceutical, Biomedical and Veterinary Sciences, University of Antwerp, Universiteitsplein 1, 2610, Antwerp, Belgium |
| Abstract: | Maesa perlarius is a medicinal plant that produces maesabalides, which possess selective and strong anti-leishmania activity. In this study, M. perlarius plants were regenerated from leaf-derived calli. Shoots were induced in Murashige and Skoog medium in the presence of thidiazuron in combination with α-naphthalene acetic acid. In contrast to seed-derived plants, callus-derived regenerants were tetraploid showing typical characteristics of higher ploidy phenotypes. We assessed the impact of indirect plant regeneration and associated increase in ploidy on the production of saponin by means of LC–MS analysis. Tetraploid M. perlarius produce a saponin profile, which was not significantly different from seed grown plants. Based on this study, we concluded that saponin production in M. perlarius is not qualitatively changed by a genome-doubling event. |
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