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Morphological studies on endocytosis of chondroitin sulphate proteoglycan by rat liver endothelial cells |
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| Authors: | Bård Smedsrød Marie Malmgren Jan Ericsson Torvard C. Laurent |
| Affiliation: | (1) Department of Medical and Physiological Chemistry, Uppsala University, Uppsala, Sweden;(2) Department of Pathology, Uppsala University, Uppsala, Sweden;(3) Department of Medical and Physiological Chemistry, Uppsala University, The Biomedical Center, Box 575, S-75123 Uppsala, Sweden |
| Abstract: | Summary Endocytosis via the hyaluronic acid/chondroitin sulphate receptor of rat liver endothelial cells was studied ultrastructurally, by use of a probe consisting of chondroitin sulphate proteoglycan attached to 15-nm gold particles. The probe bound to the surface of the cells exclusively in coated regions of the plasma membrane. Internalization at 37° C took place in less than one minute during which time interval the bound probe was transferred to coated vesicles. Further transfer to lysosomes was delayed in association with an accumulation of probes in a prelysosomal compartment consisting of large vacuoles in which probes lined the inner aspect of the membrane. Transport to lysosomes occurred only after a lag phase of at least 40–60 min at 37° C.Abbreviations CS chondroitin sulphate - CSPG chondroitin sulphate proteoglycan - CSPG-Au CSPG-gold complex - EM electronmicroscopical or electron microscopy - HA hyaluronic acid - KC Kuppfer cells - LEC liver endothelial cells - PC parenchymal cells - RES reticuloendothelial system |
| Keywords: | Receptor-mediated endocytosis Chondroitin sulphate Colloidal gold Liver endothelial cells Rat |
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