CASK inhibits ECV304 cell growth and interacts with Id1 |
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Authors: | Qi Jie Su Yongyue Sun Rongju Zhang Fang Luo Xiaofeng Yang Zongcheng Luo Xiangdong |
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Institution: | Burn Research Institute, Southwest Hospital, Third Military Medical University, State Key Laboratory of Trauma, Burn and Combined Injury, Chongqing 400038, PR China. |
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Abstract: | Calcium/calmodulin-dependent serine protein kinase (CASK) is generally known as a scaffold protein. Here we show that overexpression of CASK resulted in a reduced rate of cell growth, while inhibition of expression of endogenous CASK via RNA-mediated interference resulted in an increased rate of cell growth in ECV304 cells. To explore the molecular mechanism, we identified a novel CASK-interacting protein, inhibitor of differentiation 1 (Id1) with a yeast two-hybrid screening. Furthermore, endogenous CASK and Id1 proteins were co-precipitated from the lysates of ECV304 cells by immunoprecipitation. Mammalian two-hybrid protein-protein interaction assays indicated that CASK possessed a different binding activity for Id1 and its alternative splicing variant. It is known that Id proteins play important roles in regulation of cell proliferation and differentiation. Thus, we speculate that the regulation of cell growth mediated by CASK may be involved in Id1. Our findings indicate a novel function of CASK, the mechanism that remains to be further investigated. |
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Keywords: | CASK Id1 Cell growth Protein-protein interaction Yeast two-hybrid screening Mammalian two-hybrid assay Binding activity RNA interference ECV304 cell line MAGUK family |
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