Effect of steroid hormones on vaginal epithelial cells: Anin vitro model for steroid hormone action |
| |
Authors: | S Vijayasaradhi A Khar P D Gupta |
| |
Institution: | (1) Centre for Cellular and Molecular Biology, 500 007 Hyderabad, India |
| |
Abstract: | Conditions have been standardized to maintain rat vaginal epithelial cellsin vitro with more than 95% viability. Cultured epithelial cells were used to study the effects of normal fetal calf scrum, estradiol
and progesterone on the incorporation of 3H]-uridine in RNA and incorporation of 14C]-aminoacids in proteins. While fetal calf serum and estradiol stimulate the incorporation of both uridine and afno acids,
progesterone did not show any effect. Estradiol treated vaginal cells show typical fcroridges (indicative of keratinization
of cells) in contrast to estradiol deprived cells, which show microvilli on cell surface when examined in scanning electron
microscope. |
| |
Keywords: | Steroid hormones vaginal epithelial cells fetal calf serum |
本文献已被 SpringerLink 等数据库收录! |