Quantitative imaging of metals in tissues |
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Authors: | Martina Ralle Svetlana Lutsenko |
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Institution: | (1) Department of Biochemistry and Molecular Biology, Oregon Health & Science University, 3181 SW Sam Jackson Park Rd, Portland, OR 97239-3098, USA |
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Abstract: | Metals and other trace elements play an important role in many physiological processes in all biological systems. Characterization
of precise metal concentrations, their spatial distribution, and chemical speciation in individual cells and cell compartments
will provide much needed information to explore the metallome in health and disease. Synchrotron-based X-ray fluorescent microscopy
(SXRF) is the ideal tool to quantitatively measure trace elements with high sensitivity at high resolution. SXRF is based
on the intrinsic fluorescent properties of each element and is therefore element specific. Recent advances in synchrotron
technology and optimization of sample preparation have made it possible to image metals in mammalian tissue with submicron
resolution. In combination with correlative methods, SXRF can now, for example, determine the amount and oxidation state of
trace elements in intra-cellular compartments and identify cell-specific changes in the metal ion content during development
or disease progression. |
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Keywords: | Synchrotron-based X-ray fluorescence Metallome Metal imaging Elemental maps Submicron resolution |
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