A monoclonal antibody that detects vimentin-related proteins in invertebrates |
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| Authors: | Marika F. Walter Harald Biessmann |
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| Affiliation: | (1) Max-Planck-Institut für Virusforschung, Abteilung Physikalische Biologie, Spemannstr. 35, D74 Tübingen, Germany;(2) Present address: Department of Zoology, University of California, 95616 Davis, CA, USA;(3) Department of Biochemistry and Biophysics, University of California, 94143 San Francisco, CA, USA |
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| Abstract: | Summary Drosophila melanogaster contains a 46 000 MW cytoplasmic protein which is immunologically related to the intermediate filament protein vimentin of vertebrates. A monoclonal antibody raised against this protein was used to study its cross-reactivity with other vertebrate and invertebrate cells. Indirect immunofluorescence showed filamentous meshworks in all species tested. Protein blotting was used to determine the molecular weights of the proteins responsible for the wide range of cross-reactivity of this antibody. We present evidence that vimentin-like proteins are also present in invertebrates and form a cytoplasmic network in Paramecium. Furthermore, we demonstrate in vertebrates and invertebrates the presence of high molecular weight polypeptides which are immunologically related to vimentin. |
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