Secoiridoid content of Blackstonia perfoliata in vivo and in vitro |
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| Authors: | Aneta Sabovljevic Nedeljka Rosic Teodora Jankovic Dragoljub Grubisic |
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| Institution: | (1) Institute of Botany and Botanical Garden, Faculty of Biology, University of Belgrade, Takovska 43, 11000 Belgrade, Serbia and Montenegro;(2) Institute for Biological Research, Bulevar Despota Stefana 142, 11000 Belgrade, Serbia and Montenegro;(3) Institute of Botany, University of Cologne, Gyrhofstr. 15, 50931 Cologne, Germany |
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| Abstract: | Summary This study reports the analysis of secondary metabolites of gentiopicrin, swertiamarin, and sweroside in shoot and root cultures
of yellow wort (Blackstonia perfoliata), which were initiated from seeds, grown on Murashige and Skoog (MS) medium. Shoot cultures of B. perfoliata were inoculated with suspension of Agrobacterium rhizogenes strain A4M70GUS and hairy roots appeared at the infected sites after 3 wk of inoculation. Tips of adventitious roots of B. perfoliata were grown on hormone-free MS medium and three clones of the transformed roots regenerated shoots spontaneously. Gentiopicrin,
swertiamarin, and sweroside were detected in both roots and shoots of B. perfoliata in vitro and in vivo, but gentiopicrin was found to be the major compound. The concentration of growth regulator in the medium affected the production
of secoiridoids in B. perfoliata in vitro, where the level of gentiopicrin was higher in plants grown in the presence of indole-3-butyric acid, but the presence of
6-benzylaminopurine was inhibitory to secoiridoid production. |
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| Keywords: | gentiopicrin root and shoot culture sweroside swertiamarin transformed roots and shoots |
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