Excision of chromosomal DNA loops by treatment of permeabilised cells with Bal 31 nuclease |
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| Authors: | Maria A. Lagarkova Olga V. Iarovaia Sergey V. Razin |
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| Affiliation: | (1) Institute of Gene Biology, Russian Academy of Sciences, Vavilov St. 34/5, 117334 Moscow, Russia;(2) International Centre for Genetic Engineering and Biotechnology, Padriciano 99, I-34012 Trieste, Italy |
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| Abstract: | The specificity of long-range fragmentation of human nucleolar genes by Bal 31 nuclease was studied using fractionation of cleavage products by pulsed field gel electrophoresis, followed by Southern hybridization. It was found that limited treatment of permeabilised cells with this nuclease results in accumulation of DNA scissions in matrix attachment areas. Consequently, chromosomal DNA loops and their oligomers are released from the genome. |
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| Keywords: | Nuclear matrix DNA loops Nucleolar genes Long-range genome fragmentation Nuclease Bal 31 |
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