Soluble calcium-binding proteins: parvalbumins and calmodulin from eel skeletal muscle |
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Authors: | I Dubois C Gerday |
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Institution: | Institut de Chimie (B6) au Sart Tilman, Université de Liège, Belgium. |
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Abstract: | 1. Eel skeletal muscle contains three parvalbumin isoforms. The overall parvalbumin concentration in the muscle is 0.5 mmol kg-1 wet weight. 2. Calmodulin (0.1 mumol kg-1 wet weight) was purified by extraction with ethylenediamine tetraacetate-containing buffer, fractionation with trichloroacetic acid and separation by ion exchange chromatography on DEAE-cellulose and by molecular sieving on Ultrogel AcA 54. 3. Troponin-C-free calmodulin was obtained by fast protein liquid chromatography on a Mono Q column. |
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