Interaction of NMDA and Dopamine D2L Receptors in Human Neuroblastoma SH-SY5Y Cells |
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| Authors: | Venugopalan D. Nair Hyman B. Niznik Ram K. Mishra |
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| Affiliation: | Departments of Psychiatry and Biomedical Sciences, McMaster University, Hamilton;and; Laboratory of Molecular Neurobiology, The Clark Institute of Psychiatry, Toronto, Ontario, Canada |
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| Abstract: | Abstract: To understand the mechanism of interaction of the dopamine D2L receptors with NMDA receptors, we have developed a model by transfecting human neuroblastoma SH-SY5Y cells with the human dopamine D2L receptor gene. In vitro blockade of NMDA receptors by the specific antagonists MK-801 and (±)-3-(2-carboxypiperazin-4-yl)-propyl-1-phosphonic acid (CPP) on human neuroblastoma SH-SY5Y cells expressing human dopamine D2L receptors resulted in a significant increase in the density of D2L receptors without a significant change in receptor affinity. Moreover, the dopamine receptor mRNA level increased by ∼50% by the blockade of NMDA with MK-801. These results suggest a possible interaction of NMDA and dopamine D2L receptors in neuroblastoma SH-SY5Y cells. This system would serve as an excellent model to study the molecular mechanisms involved in the interaction of these two receptors. |
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| Keywords: | Dopamine receptors mRNA Neuroblastoma NMDA receptors Transfection |
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