Enhancement of detection for partially methylated alditol acetates by chemical ionization mass spectrometry |
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Authors: | R A Laine |
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Institution: | 1. Department of Research, St. Elizabeth''s Hospital, 736 Cambridge Street, Boston, Massachusetts 02135 USA;2. Division of Hematology-Oncology, St. Elizabeth''s Hospital, 736 Cambridge Street, Boston, Massachusetts 02135 USA;3. the Department of Medicine, Tufts University School of Medicine, Boston, Massachusetts 02111 USA |
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Abstract: | Treatment of washed, intact platelets with Bolton-Hunter reagent is a satisfactory method for 125I-labeling of many platelet proteins. Analysis by two dimensional polyacrylamide gel electrophoresis and autoradiography shows that the major platelet cytoskeletal proteins and at least four surface-exposed proteins are labeled. The method allows the identification of these labeled proteins in amounts that are below the limits of detection by Coomassie blue staining. Two granule proteins, thrombospondin and fibrinogen, are slightly labeled. Conditions of labeling do not appear to affect platelet structure or function, as assessed by phase-contrast microscopy, 51CrO42? release, and aggregation in response to thrombin or fibrinogen/adenosine-5′-diphosphate. |
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