Lignin transformation by a versatile peroxidase from a novel Bjerkandera sp. strain |
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| Affiliation: | 1. Departamento de Biotecnologia, Instituto Nacional de Engenharia, Tecnologia e Inovação (INETI), Estrada do Paço do Lumiar, 22, P-1649-038 Lisboa, Portugal;2. Escola Superior de Biotecnologia, Universidade Católica Portuguesa, R. Dr. António Bernardino de Almeida, P-4200-072 Porto, Portugal;1. Department of Food and Environmental Sciences, Viikki Biocenter, University of Helsinki, Finland;2. School of Biology, University of Nottingham, UK;3. Department of Bio- and Environmental Sciences, International Graduate School of Zittau, Germany;1. Centro de Investigaciones Biológicas, CSIC, Ramiro de Maeztu 9, E-28040 Madrid, Spain;2. TU Dresden – International Institute Zittau, Markt 23, 02763 Zittau, Germany;1. Department of Medicinal and Applied Chemistry, Kaohsiung Medical University, Kaohsiung City, Taiwan;2. Research Center for Environmental Medicine, Kaohsiung Medical University, Kaohsiung City, Taiwan;3. Department of Environmental Energy Engineering, Kyonggi University, Suwon, Republic of Korea;4. Division of Chemistry, Faculty of Science and Humanities, Sree Sowdambika College of Engineering, Aruppukottai, Tamil Nadu, India;5. Department of Chemistry and Research Centre, Aditanar College of Arts and Science, Virapandianpatnam, Tiruchendur, Tamil Nadu, India;6. Department of Civil Engineering, Regional Campus Anna University Tirunelveli, Tamilnadu, India;7. Research Institute of Biotechnology and Medical Converged Science, Dongguk University, Seoul, Ilsandong–gu, Goyang–si, Gyeonggi–do 10326, Republic of Korea;8. Green Processing, Bioremediation and Alternative Energies Research Group, Faculty of Environment and Labour Safety, Ton Duc Thang University, Ho Chi Minh City, Viet Nam;1. Ministry of Education Key Laboratory of Cell Activities and Stress Adaptations, School of Life Science, Lanzhou University, Tianshuinanlu #222, Lanzhou, Gansu 730000, People’s Republic of China;2. Institute for Energy Research, School of the Environment and Safety Engineering, Jiangsu University, Zhenjiang, Jiangsu 212013, People’s Republic of China;1. TU Dresden, International Institute Zittau, Department of Bio- and Environmental Sciences, Markt 23, 02763 Zittau, Germany;2. University of Buenos Aires, Faculty of Exact and Natural Sciences, Department of Biodiversity and Experimental Biology, Intendente Güiraldes 2160, Ciudad Universitaria, C1428EGA, Argentina;3. Interfaculty Institute for Genetics and Functional Genomics, Department of Functional Genomics, Ernst Moritz Arndt University of Greifswald, Greifswald, Germany |
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| Abstract: | A versatile peroxidase, purified from a novel strain of Bjerkandera sp. (B33/3), was tested for its reactivity on a lignin fraction obtained from straw pulping. The effects of such processing parameters as reaction time, pH, and lignin:enzyme ratio were evaluated. Gel filtration chromatography was employed to characterise the molecular mass distribution of the lignin fragments produced by the enzyme-mediated reaction. Our results have shown that such a versatile peroxidase can directly bring about transformations of lignin, even in the absence of external mediators. |
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