发热伴血小板减少综合征布尼亚病毒规模化生产培养工艺的建立

目的通过对发热伴血小板减少综合征布尼亚病毒（简称“新布尼亚病毒”）进行规模化生产培养工艺研究，为新布尼亚病毒规模化生产提供有力支持。方法采用细胞工厂培养Vero细胞，待其长成致密单层后，取工作种子批新布尼亚病毒毒种接种细胞，采用连续收获或细胞病变充分时收获培养液上清的方法收获病毒，并以病毒滴度、抗原含量作为评价指标选择基础培养基、培养基pH、人血白蛋白添加浓度、接种细胞日龄、接种病毒MOI以及病毒培养温度。结果按0．01-0．001MOI接种3-4日龄Vero细胞，病毒培养液选择含0．3％人血白蛋白pH7．6-7．8的DMEM溶液，35℃培养7d收获，病毒收获液病毒滴度7．87LgCCID50／mL、抗原含量170．1μ/mL。结论初步建立了新布尼亚病毒规模化生产培养工艺，为后续工业化生产提供了数据支持。

Establishment of the culture process for scale production of SFTS Bunyavirus

Objective To investigate the ideal culture process of SFTS Bunyavirus and provide the basic date for scale pro- duction. Methods Vero cells were cultured using cell factory. The working seed of SFTS bunyavirus was inoculated into Vero cells when the cells grew into monolayer. The virus were harvested with continuous perfused culture system or harves- ting the supernatant of culture when the cells achieved fully lesions. The virus titers and antigen contents were used as indi- cators for selecting medium, pH of medium, added human serum albumin concentration, virus incubation temperature, cells age and MOI of virus. Results 3-4 days old Vero cells were inoculated with 0.01-0. 001, MOI of SFTS Bunyavirus, DMEM solution containing 0.3% human serum albumin was selected as the medium （ pH 7.6-7.8 ）, the virus were cul- tured at 35 ~C for 7 days, the harvested virus titer is 7.87 LgCCIDso/mL and the antigen content is 170.1 ixg/mL. Con- clusion The scale culture process of SFTS Bunyavirus was initially established, and it laid a good foundation for industrial production.