肺炎克雷伯杆菌疫苗中试生产中细菌培养工艺的改进及优化

目的中试生产中对肺炎克雷伯杆菌培养工艺进行改进及优化。方法采用液体综合培养基代替半综合培养基在10L和100L中国丽生物反应器中对肺炎克雷伯杆菌进行培养，在10L中国丽生物反应器探讨不同的培养基配方、pH值、培养温度、搅拌转速、溶氧，工艺参数稳定后，扩大培养到100L中国丽生物反应器，并探讨培养过程中补加葡萄糖的浓度及补加方式等对细菌浓度及荚膜多糖含量的影响。结果肺炎克雷伯杆菌液体综合培养基可代替半综合培养基用于该菌的培养，培养过程中维持pH值7．2、温度37℃、通气60L／h、搅拌转速250r／min、培养到2h时开始以恒速补加30mL／L40％葡萄糖溶液、培养时间为5h，细菌长势最好，收获的荚膜多糖含量最高。结论肺炎克雷伯杆菌的培养工艺放大到100L中国丽生物反应器中，经过多次试验初步建立了稳定的肺炎克雷伯杆菌中试培养工艺。

Improvement and optimization of bacterial culture process in Klebsiella pneumoniae vaccine pilot scale production

Objective To improve the cuhural process and select the optimity for producing Klebsiella pneumoniae vaccine. Methods The integrated liquid medium was instead of semi-integrated medium for inoculating Klebsiella pneumoniae in bioreactor （ 10 L and 100 L of volume） , and the effects of various factors （ such as medium formulation, pH, culture tem- perature, stirring speed, oxygen concentration, supplemented glucose content and its supplementing time pon ） on the growth and polysaccharide yield were detected in bioreactor. Results The results showed that the integrated liquid medium can be used for inoculating Klebsiella pneumoniae to substitute for semi-synthetic medium. The optimum culture conditions are as follows ： pH 7.2, temperature at 37 ~C , stirring speed at 250 r/m, 60 L/h ventilation, and supplementing 3 000 mL of 40% glucose solution into 100 L medium after inoculating two hours, and then continuously cultured for three hours, by this time the growth state of the bacteria is the best, polysaccharrde content is the highest. Conclusion Stable Klebsiella pneumonia fermentation for producin~ vaccine has been established by several validated test with 100 L bioreactor.