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促肾上腺皮质激素对慢痛大鼠脊髓生长抑素、c-fos表达及痛反应的影响
作者姓名:Wu Q  Li XC  Ruan HZ  Li HD
作者单位:第三军医大学生理教研室,重庆,400038
摘    要:本研究应用免疫组化、原位杂交和痛级均数测定法,探讨鞘内注射促肾上腺皮质激素(ACTH)对甲醛引起大鼠脊髓内生长抑素(Som)、cfos表达及痛反应的影响。结果表明,足底注射甲醛可使大鼠脊髓内cfos样免疫反应(FLI)、Som样免疫反应(SomLI)、FLI/SomLI及前Som原mRNA(PPSmRNA)神经元数目显著增多以及痛级均数(PIR)显著升高。而鞘内注射ACTH可显著抑制甲醛引起的大鼠脊髓内FLI、SomLI、FLI/SomLI及PPSmRNA增多和PIR升高效应。鞘内预先注射赛庚啶可阻断ACTH的抑制效应,而荷包牡丹碱、纳洛酮则无影响。结果提示,5羟色胺受体可能参与ACTH抑制甲醛引起的痛反应。

关 键 词:ACTH  生长抑素  原癌基因c-fos  脊髓  免疫组化

Effect of ACTH on the expression of somatostatin and c-fos in the spinal cord and formalin evoked pain response
Wu Q,Li XC,Ruan HZ,Li HD.Effect of ACTH on the expression of somatostatin and c-fos in the spinal cord and formalin evoked pain response[J].Acta Physiologica Sinica,1999,51(1):60-64.
Authors:Wu Q  Li X C  Ruan H Z  Li H D
Institution:Department of Physiology, Third Military Medical University, Chongqing 400038.
Abstract:In the present study, the effect of ACTH on the expression of somatostatin and c fos in the spinal cord and formalin induced pain responses in rats were studied using immunohistochemistry and in situ hybridization. The results showed that subcutaneous injection of formalin in the right hindpaw increased c fos like immunoreactivity (FLI), somatostatin like immunoreactivity (Som LI), Som LI/FLI and perprosomatostatin mRNA (PPS mRNA) in neurones of right spinal dorsal horn and significantly enhanced pain intensity rating. ACTH decreased the FLI, Som LI, Som LI/FLI and PPS mRNA levels of the spinal cord evoked by formalin. The decrease of c fos or Som level due to intrathecal injection of ACTH in rats with chronic pain was prevented by injection of cyproheptadine, but not by bicuculline and naloxone. The results indicate that the serotonin receptor may be involved in ACTH induced analgesia.
Keywords:ACTH  somatostatin  proto  oncogene  c  fos  spinal cord  immunohistochemistry  in situ    hybridization  
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