Protein polymorphism in native goats from central Argentina |
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| Affiliation: | 1. Facultad de Ciencias Agropecuarias, Universidad Nacional de Córdoba, Casilla de Correo 509, 5000 Córdoba, Argentina;2. Cátedra de Quı́mica Biológica, Facultad de Ciencias Médicas, Universidad Nacional de Córdoba, Casilla de Correo 35 Sucursal 16, 5000 Córdoba, Argentina;3. Cátedra de Fisiologı́a Humana, Facultad de Ciencias Quı́micas, Universidad Nacional de Córdoba, Casilla de Correo 61, 5000 Córdoba, Argentina;1. Key Laboratory of Imaging Diagnosis and Minimally Invasive Intervention Research, The Fifth Affiliated Hospital of Wenzhou Medical University, Affiliated Lishui Hospital of Zhejiang University, The Central Hospital of Zhejiang Lishui, Lishui, 323000, China;2. Department of Radiology, The Fifth Affiliated Hospital of Wenzhou Medical University, Affiliated Lishui Hospital of Zhejiang University, The Central Hospital of Zhejiang Lishui, Lishui, 323000, China;1. Institut National de la Recherche Agronomique, Unité de Recherches Zootechniques, Domaine Duclos, 97170 Petit-Bourg (French West Indies), France;2. Institut National de la Recherche Agronomique, Domaine expérimental de Gardel, 97160 Moule, Guadeloupe, France;1. Department of Ophthalmology, Sorbonne Université, Pitié-Salpêtrière Hospital, Assistance Publique-Hôpitaux de Paris (AP-HP), Paris 75013, France;2. Department of Ophthalmology, Université de Paris, Paris Descartes Faculty of Medicine, Cochin Hospital, AP-HP, Paris, France;3. Department of Ophthalmology, Centre Hospitalier Universitaire de Bordeaux, Institut National de la Santé et de la Recherche Médicale (INSERM), Bordeaux Population Health Research Center, Bordeaux, France;4. Department of Ophthalmology, Sorbonne Université, Centre Hospitalier National d''Ophtalmologie des Quinze-Vingts, INSERM, CNRS, Institut de la Vision, Paris, France;5. Department of Ophthalmology, Nantes University Hospital, Nantes, France;6. Department of Ophthalmology, Felix Guyon University Hospital, Saint Denis (La Réunion), France;7. HP2 Laboratory, INSERM U1042 Unit, Grenoble-Alpes University, Grenoble, France;8. Department of Ophthalmology, Université de Reims Champagne Ardenne, Reims, France;9. Department of Ophthalmology, Toulouse University Hospital, Toulouse, France;10. Department of Ophthalmology, Montpellier University Hospital, Montpellier, France;11. Department of Ophthalmology, Rennes University Hospital, Rennes, France;12. Department of Ophthalmology, Saint-Etienne University Hospital, Saint-Etienne, France;13. Fondation Ophtalmologique Rothschild, Paris, France;14. Université de Paris, Bichat Hospital, AP-HP, Paris, France;15. Hospices Civils de Lyon, Edouard Herriot University Hospital, Lyon, France;p. Croix Rousse University Hospital, Lyon, France;q. Department of Ophthalmology, The University of Pittsburgh School of Medicine, Pittsburgh, PA, USA;r. Ophthalmology Department, Université de Paris, AP-HP, Hôpital Lariboisière, Paris, France;1. Department of Microbiology, Faculty of Science, Mahidol University, Rama 6 Road, Bangkok 10400, Thailand;2. Ministry of Health, Private Bag 0038, Gaborone, Botswana;3. Mahidol University, Amnatcharoen Campus, Muang, Amnatcharoen 37000, Thailand;4. National Center for Genetic Engineering and Biotechnology, National Science and Technology Development Agency, Pathumthani 12120, Thailand;1. Escola de Engenharia de Lorena, Universidade de São Paulo, Estrada Municipal do Campinho S/N, CEP 12602-810, Lorena, SP, Brazil;2. Universidade Federal da Fronteira Sul, Campus Chapecó, CEP 89802-112, SC, Brazil;3. Instituto de Física, Universidade Federal de Mato Grosso, CEP 78060-900, Cuiabá, MT, Brazil |
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| Abstract: | Under the name of Creole goats, individuals of various origins, morphological and productivity characters are grouped. To characterize the genetic pool of goats from different sources in central Argentina, protein polymorphism revealed by electrophoresis was analyzed. A total of 109 adult animals from Santa Marı́a, Colón and Ischilı́n Districts, Córdoba Province, were studied. The red cells lysates were subjected to starch gel electrophoresis and specific staining procedures to reveal the activity of different enzymes and hemoglobin. Separation of plasmatic proteins was carried out by electrophoresis in polyacrylamide gel and stained with Coomassie Blue. From a total of 14 loci analyzed, eight were polymorphic in at least one flock. Four codominant alleles were detected in the locus βHemoglobin (βHb); three alleles in malic enzyme (Me), glucose 6 phosphate dehydrogenase (G6pdh) and lactate dehydrogenase (Ldh) and two alleles in catalase (Cat), transferrin (Tf), esterase-2 (Es-2) and phosphoglucomutase (Pgm). In all the cases, the observed genotype frequencies were not significantly different from those expected from Hardy–Weinberg equilibrium. The proportion of polymorphic loci (P%) varied between 21.4% and 42.9% and mean heterozygosity (H), between 0.061 and 0.117. Alleles at loci Cat, Me and Es-2 showed significantly different frequencies according to the sample origin. The flock from Colón District presented the highest levels of polymorphism. This is the only stock which includes hybrids of varying grade between Creole stocks and male specimens from Saanen and Nubian breeds. |
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