| 里氏木霉和鸡腿菇利用秸秆共发酵产木质降解酶 |
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| 引用本文： | 葛春梅,徐娟娟,孙芹英,张洁,蔡敬民,潘仁瑞. 里氏木霉和鸡腿菇利用秸秆共发酵产木质降解酶[J]. 生物工程学报, 2009, 25(12): 2008-2013 |
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| 作者姓名： | 葛春梅 徐娟娟 孙芹英 张洁 蔡敬民 潘仁瑞 |
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| 作者单位： | 1. 合肥学院生物与环境工程系,合肥,230022
2. 合肥学院生物与环境工程系,合肥,230022;安徽农业大学微生物防治省重点实验室,合肥,230036 |
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| 基金项目： | 安徽省自然科学基金项目(No.070413266X);;安徽省教育厅优秀人才基金(No.2009SQR2162);;合肥学院博士基金项目(No.RC0016);;合肥学院自然科学基金项目(No.09KY11ZR)资助~~ |
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| 摘 要： | 为了更好地利用农业废弃物,提高其综合利用率,减少传统化学方法及秸秆焚烧过程造成的环境污染,实验对鸡腿菇、黑曲霉和里氏木霉3株产木质纤维素降解酶系的菌株进行混合平板产酶筛选,结果显示鸡腿菇和里氏木霉平板培养相容性良好,且产酶量高。在相容性实验的基础上,对鸡腿菇和里氏木霉的最优产酶条件进行了研究。在最优条件下:鸡腿菇和里氏木霉接种比例按5:2,接种时间间隔为12h,26oC、150r/min下,发酵3d产漆酶活力达3267.2U/mL,比单独发酵提高106%。
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| 关 键 词： | 鸡腿菇 里氏木霉 木质纤维素酶 漆酶 共发酵 |
| 收稿时间： | 2009-09-24 |
Production of ligninase by co-fermentation of Coprinus comatus and Trichoderma reesei |
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| Chunmei Ge,Juanjuan Xu,Qinying Sun,Jie Zhang,Jingmin Cai and Renrui Pan. Production of ligninase by co-fermentation of Coprinus comatus and Trichoderma reesei[J]. Chinese journal of biotechnology, 2009, 25(12): 2008-2013 |
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| Authors: | Chunmei Ge Juanjuan Xu Qinying Sun Jie Zhang Jingmin Cai Renrui Pan |
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| Affiliation: | Department of Biology & Environment Engineering, Hefei University, Hefei 230022, China;Department of Biology & Environment Engineering, Hefei University, Hefei 230022, China; Anhui Provincial Key Laboratory of Microbial Pest Control, Anhui Agricultural University, Hefei 230036, China;Department of Biology & Environment Engineering, Hefei University, Hefei 230022, China;Department of Biology & Environment Engineering, Hefei University, Hefei 230022, China;Department of Biology & Environment Engineering, Hefei University, Hefei 230022, China;Department of Biology & Environment Engineering, Hefei University, Hefei 230022, China |
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| Abstract: | In order to enhance the utilization efficiency,reduce the environmental pollution of traditional chemical treatment and the agriculture waste incineration;we studied the ligninase production by Coprinus comatus,Aspergillus niger and Trichoderma reesei through the plate screening.The results showed that C.comatus mixed culture with T.reesei have a good compatibility and higher yields of Laccase.On the basis of this pre-experiment,we studied the optimal conditions of mixed culture for enzyme production.Under ... |
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| Keywords: | Coprinus comatus Trichoderma reesei lingo-cellulase laccase co-fermentation |
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