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Sequence-Specific Alkylation of DNA by Duocarmycin A and Its Novel Derivatives Bearing PY/IM Polyamides |
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| Authors: | Z. F. Tao T. Fujiwara I. Saito H. Sugiyama |
| Affiliation: | 1. Institute for Medical and Dental Engineering, Tokyo Medical and Dental University , Tokyo, 101-0062, Japan;2. Department of Synthetic Chemistry and Biological Chemistry , Faculty of Engineering, Kyoto University , Kyoto, 606-8501, JapanCREST, JST |
| Abstract: | Abstract A new class of sequence-specific DNA alkylating agents were developed based on the reactivity of duocarmycin A and the DNA-reading ability of pyrrole-imidazole polyamide. The DNA alkylation sequence specificity by duocarmycin A can be modulated by a variety of pyrrole-imidazole triamides in a predictable manner. Novel hybrids of the segment A of duocarmycin A and pyrrole-imidazole polyamides efficiently and highly selectively alkylated the target base possessing match sequences of Dervan's binding code. |
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