Cleavage Of Oligodeoxyribonucleotides From Polymer Supports And Their Rapid Deprotection Under Microwaves |
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Authors: | K C Gupta P Kumar |
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Institution: | Nucleic Acids Research Laboratory, Centre for Biochemical Technology , Mall Road, Delhi University Campus, Delhi , 110 007 , India |
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Abstract: | Abstract Novel conditions for the cleavage of oligodeoxynucleotides from polymer supports and their complete deprotection under microwaves have been developed. The oligonucleotides synthesized using phosphoramidite synthons carrying base labile (Pac, Dmf and t-Bpac) and conventional (Bz for A and C and Pac for G) protecing groups for nucleic bases were deprotected using 0.2M sodium hydroxide (MeOH : H2O:: 1:1, v/v) = Reagent A and 1M sodium hydroxide (MeOH: H2O :: 1:1, v/v = Reagent B, respectively under microwaves. The deprotected oligonucleotides were found to be comparable with the corresponding oligonucleotides deprotected under standard conditions (aq. ammonia at 55°C). |
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