Effect of Oligomer Length and Base Substitutions on the Cytotoxic Activity and Specific Nuclear Protein Recognition of GTn Oligonucleotides in the Human Leukemic CCRF-CEM Cell Line |
| |
| Authors: | Carla Morassutti Barbara Dapas Bruna Scaggiante Gabriela Paroni Luigi Xodo Franco Quadrifoglio |
| |
| Affiliation: | Department of Biomedical Sciences and Technologies , University of Udine , via Gervasutta, 48-33100, Udine, Italy |
| |
| Abstract: | Abstract We have identified phosphodiester oligonucleotides composed of G and T bases, named GTn, which are able to inhibit the cellular growth of human cancer cell lines by recognising specific nuclear proteins. We demonstrated that GTn oligonucleotides require a length of at least 20 nucleotides in order to exert a significant cytotoxic effect and to retain the specific protein binding ability. In addition, we found that GTn cytotoxicity was lost when A or C bases were introduced at either 3′ and 5′end or within the GTn sequences. |
| |
| Keywords: | |
|
|
