Synthesis of A Polyaminooligonucleotide Combinatorial Library |
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| Authors: | Wojciech T Markiewicz Przemyslaw Godzina Maria Markiewicz Anna Astriab |
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| Institution: | Institute of Bioorganic Chemistry, Polish Academy of Sciences , Noskowskiego 12, PL-61704 , Poznan , Poland |
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| Abstract: | Abstract A new method of synthesis of phosphoramidite of 2′-deoxycytydine carrying a protected spermine moiety at N-4 position is described. A model oligodeoxyribonucleotide containing the specified nucleoside unit has been synthesised. A synthesis of a polyaminooligonucleotide combinatorial library was carried out. The analysis of the above library clearly shows that the presence of spermine moieties in oligodeoxyribonucleotides increases stability of their duplexes. |
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