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Identification of a Good C-MYC Antisense Oligodeoxynucleotide Target Site and the Inactivity at This Site of Novel NCH Triplet-Targeting Ribozymes |
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| Authors: | Richard V Giles David G Spiller Richard E Clark David M Tidd |
| Institution: | 1. School of Biological Sciences, University of Liverpool , Life Science Building, Crown Street, Liverpool, L69 7ZB;2. Department of Haematology , Royal Liverpool University Hospital , Prescot street, Liverpool, L7 8XP;3. Department of Haematology , Royal Liverpool University Hospital , Prescot street, Liverpool, L7 8XP |
| Abstract: | Abstract A region of c-myc mRNA was identified which permitted very efficient antisense effects to be achieved in living cells using chimeric methylphosphonate-phosphodiester antisense effectors. Novel inosine—containing ribozymes (which cleave after NCH triplets) were directed to an ACA triplet within this region and delivered into living cells. No ribozyme intracellular activity could be identified. Very low ribozyme function was also observed in in vitro assays using a 1700nt substrate RNA. |
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