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Synthesis and Biological Evaluation of Modified DNA Fragments for The Study of Nucleotide Excision Repair in E. coli |
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| Authors: | Vania Monaco Karin I van de Wetering Nico J Meeuwenoord Hans A van den Elst Hanneke R Stuivenberg Rob Visse |
| Institution: | 1. Leiden Institute of Chemistry;2. Laboratory of Molecular Genetics, Leiden Institute of Chemistry, Gorlaeus Laboratories , P. O. Box 9502, 2300, RA Leiden, The Netherlands |
| Abstract: | Abstract Three new cholesterol-containing phosphoramidites where synthesized and used in automated synthesis of modified DNA fragments. These cholesterol lesions are good substrates for the E. coli UvrABC endonuclease. In vitro they are incised from damaged DNA with higher efficiency in respect with the cholesterol lesions previously published. |
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