Interactions of High Affinity Anti (6-4) Photoproduct Antibody Fragments with Damaged DNA |
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| Authors: | Eiko Ohtsuka Hiroyuki Kobayashi Hiroshi Morioka Kousuke Sato Yasuo Komatsu Osamu Nikaido |
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| Institution: | 1. Graduate School of Pharmaceutical Sciences, Hokkaido University , Sapporo, 060-0812, Japan;2. Faculty of Pharmaceutical Sciences, Kanazawa University , Kanazawa, Japan |
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| Abstract: | Abstract The interactions between chemically synthesized DNA fragments containing a T(6-4)T and antigen binding fragments (Fab) or single-chain antibodies (scFv) were investigated by X-ray crystallography, NMR, and surface plasmon resonance. The high affinity scFv protein was found to bind to the template DNA near the (6-4) photoproduct site and to interfere with DNA polymerase reactions in vitro. |
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