Boranophosphate Nucleic Acids - A Versatile DNA Backbone |
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| Authors: | Vladimir Rait Dmitri Sergueev Jack Summers Kaizhang He Faqing Huang Bozenna Krzyzanowska |
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| Institution: | Department of Chemistry , Duke University , Durham, NC, 27708-0346, USA |
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| Abstract: | Abstract Important chemical and biochemical properties of boranophosphate DNA and RNA oligonucleotides are reviewed. Stereoregular boranophosphate oligomers can be synthesized enzymatically and form stable duplexes with DNA. Fully boronated, non-stereoregular oligothymidylates, synthesized chemically, form hybrids with poly(A) that have lower melting points than oligothymidylate:poly(A), yet they nevertheless can support the RNase H mediated cleavage of RNA. |
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