Preparation of Stereoregulated Antisense Oligodeoxyribonucleoside Phoshorothioate and Interaction with its Complementary DNA And RNA |
| |
| Authors: | Yutaka Tamura# Hidetaka Miyoshi Tomoyuki Yokota Keisuke Makino$ Akira Murakami |
| |
| Affiliation: | 1. Department of Polymer Science and Engineering , Kyoto Institute of Technology , Matsugasaki, Sakyo-ku, Kyoto, 606, Japan;2. Rational Drug Design Laboratories , Misato, Matsukawa-Machi, Fukushima, 960-12, Japan |
| |
| Abstract: | Abstract Diastereoisomcric specificity of oligodeoxyribonucleoside phosphorothioate (OPT) in DNA/OPT and RNA/OPT hybrid formation was investigated. The difference in the configuration between RRRR and SSSS was reflected in the conformation and the stability of the DNA/OPT and RNA/OPT hybrids. Therefore, findings of this report rationalize the antisense effect by non-stereoregulated OPT and the difference of diastereoisomerism in susceptibility to RNase H. |
| |
| Keywords: | |
|
|
