Mechanism and Specificity of RNA Cleavage by Chemical Ribonucleases |
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| Authors: | N. Beloglazova A. Vlassov D. Konevetc V. Sil'nikov M. Zenkova R. Giege |
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| Affiliation: | 1. Institute of Bioorganic Chemistry , Lavrentiev ave. 8, Novosibirsk, 630090, Russia;2. Institut de Biologie Moleculaire et Cellulaire du C.N.R.S. , Strasbourg, France |
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| Abstract: | Abstract Cleaving of model RNA substrates by chemical ribonucleases constructed by conjugation of 1,4 diazabicyclo[2,2,2]octane with histamine and histidine was investigated. Similarly to RNase A, the chemical RNases produce fragments with 5′ hydroxy-group and 3′-cyclophosphate. The cleavage occurs as the catalytic reaction: more than 150 phosphodiester bonds in RNA can be cleaved by one molecule of RNase mimic. |
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