Binding of Chemically-Modified Oligonucleotides to the Double-Stranded Stem of an RNA Hairpin |
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| Authors: | Karine Aupeix Jean-Jacques Toulmé |
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| Affiliation: | INSERM U 386, IFR Pathologies Infectieuses, Université , Victor Segalen, 146 rue Léo Saignat, 33076, Bordeaux Cedex, France Fax: E-mail: jean-jacques.toulme@bordeaux.inserm.fr |
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| Abstract: | Abstract We monitored the binding of triplex-forming oligopyrimidines to the double-stranded stem of the RNA hairpin responsible for the gag-pol frameshift in HIV-1. Whereas the substitution of 5, propynyl-C for C had a limited effect, the use of a Peptide Nucleic Acid 12mer led to a drastic reduction in the stability of the oligomer/RNA complex. |
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