Separation of naturally occurring 3',5'-cyclic ribonucleotides using high pressure liquid chromatography. |
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| Authors: | S P Dutta A Mittelman G B Chhed'a |
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| Institution: | Center for Developmental Oncology Department of Surgery Roswell Park Memorial Institute Buffalo, New York 14263 USA |
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| Abstract: | A simple and fairly rapid procedure is described for separation of a mixture of five 3′, 5′-cyclic ribonucleotides. The separation was achieved by high pressure liquid chromatography on a Vydac pellicular anion exchange column using a linear gradient of 0.5 to 1.0 m acetic acid as the eluting solvent. |
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