| 来自Yeosuana marina sp.JLT21内切型海藻酸裂解酶的异源表达及酶学表征 |
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| 引用本文： | 常晴,束月蓉,王文韬,蒋昊,延泉德,钱政,高雪纯,吴金鸿,张勇. 来自Yeosuana marina sp.JLT21内切型海藻酸裂解酶的异源表达及酶学表征[J]. 生物技术通报, 2022, 0(2): 123-131 |
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| 作者姓名： | 常晴 束月蓉 王文韬 蒋昊 延泉德 钱政 高雪纯 吴金鸿 张勇 |
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| 作者单位： | 上海交通大学生命科学技术学院微生物代谢国家重点实验室;上海交通大学农业生物技术学院农业与生物学院食品科学与工程系 |
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| 基金项目： | 国家重点研发计划(2019YFD0901901);国家自然科学基金项目(31770846)。 |
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| 摘 要： | 褐藻寡糖有着丰富的生物学功能,酶法制备功能性褐藻寡糖具有重要实践应用价值.为发掘高活性及稳定性的褐藻寡糖制备酶,对浅海热液嗜热菌Yeosuana marina sp.JLT21中的海藻酸裂解酶YMA-1的基因在大肠杆菌中进行表达、纯化及酶活鉴定.结果发现YMA-1由306个氨基酸残基构成,是多糖裂解酶家族7(PL7)新...
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| 关 键 词： | 内切型海藻酸裂解酶 重组表达 酶学性质 Yeosuana marina sp.JLT21 |
Heterologous Expression and Characterization of Endo-type Alginate Lyase from Yeosuana marina sp.JLT21 |
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| CHANG Qing,SHU Yue-rong,WANG Wen-tao,JIANG Hao,YAN Quan-de,QIAN Zheng,GAO Xue-chun,WU Jin-hong,ZHANG Yong. Heterologous Expression and Characterization of Endo-type Alginate Lyase from Yeosuana marina sp.JLT21[J]. Biotechnology Bulletin, 2022, 0(2): 123-131 |
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| Authors: | CHANG Qing SHU Yue-rong WANG Wen-tao JIANG Hao YAN Quan-de QIAN Zheng GAO Xue-chun WU Jin-hong ZHANG Yong |
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| Affiliation: | (State Key Laboratory of Microbial Metabolism,School of Life Sciences and Biotechnology,Shanghai Jiao Tong University,Shanghai 200240;Department of Food Science&Technology,School of Agriculture and Biology,Shanghai Jiao Tong University,Shanghai 200240) |
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| Abstract: | Alginate oligosaccharides(AO)have rich biological activity,preparing AO using enzymatic approach is of important,practical and applicable value.To mine highly active and stable enzymes for preparing AO,a gene from an alginate lyase YAM-1 of Yeosuana marina sp.JLT21 was expressed in Escherichia coli,and the enzyme was purified and characterized.As one of polysaccharide lyase family 7(PL7)members,YMA-1 consisted of 306 amino acids.The recombinant YMA-1 showed the optimal activity of 1.3×10;U/mg at pH 9.0 and 55℃,and Cu2+efficiently stimulated its activity.YMA-1 also demonstrated a highly catalytic activity towards sodium alginate,poly G and poly M,and their specific activities were(5 201.21±86.46),(6 399.73±253.12)and(3 751.68±116.25)U/mg,respectively under 37℃ and pH 9.0.The end-products from the enzymolysis of sodium alginate were mainly unsaturated trisaccharides and tetrasaccharides.Duo to its broad substrate spectrum,high activity and thermostability,YMA-1 as endo-type alginate lyase in PL7 family has potential applications in green and efficient production of alginate oligosaccharides. |
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| Keywords: | endo-type alginate lyase recombinant expression enzymatic properties Yeosuana marina sp.JLT21 |
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