透明质酸的生物合成及其代谢工程的研究进展

透明质酸(hyaluronic acid,HA)是广泛存在于生物体内的功能性糖胺高分子聚合物,在日化、医疗和食品领域应用前景广阔.随着基因工程与代谢工程等合成生物学技术的发展,人们对HA的生物合成过程和机理解析越发深入的同时,也伴随一些新的挑战来临.该综述从分子生物学角度总结了HA的关键合成酶基因及合成途径,对不同来源...

Research Progress in the Biosynthesis and Metabolic Engineering of Hyaluronic Acid

Hyaluronic acid(HA),a functional glycosamine polymer widely existing in organism,presents broad potential applications in daily-use chemicals,medicine and food.With the development of synthetic biology technologies such as genetic engineering and metabolic engineering,some new challenges are emerging while the biosynthesis process and mechanism of HA have been studied in depth.This review summarizes the key genes and pathways of HA synthase from the perspective of molecular biology,aligns and analyses the amino acid sequence of HA synthase from different sources.It also describes in detail the bottleneck issues such as the safety of HA production,substrate imbalance and low oxygen solubility in fermentation,and introduces the development of strategies on corresponding synthetic biology in commonly used microbial hosts,thereby induces the effective strategies for high-yield HA by microorganism.This review may provide an important support for further promoting the green biomanufacturing of HA.