Quantitation of Endogenous C-type Virion Production in Several Murine Cell Lines |
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Authors: | Ronald B. Luftig Paul N. Mcmillan Mollie Gudger |
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Affiliation: | 1Worcester Foundation for Experimental Biology, Shrewsbury, Massachusetts 01545 |
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Abstract: | Quantitation by enumeration of virion particles, measurement of absorbancy at 260 nm, and densitometry on sodium dodecyl sulfate-polyacrylamide gels has shown that mouse L-M cells yielded 7- to 10-fold less endogenous C-type virions than the parental lines, L or L929. The previously noted stimulation of L-M cell virion production by a concomitant 10% increase in fetal calf serum concentration (D. A. Kindig, R. Karp, and W. H. Kirsten, 1968), was not observed. |
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