Kluyveromyces lactisKiller Plasmid pGKL2: Evidence for a Viral-like Capping Enzyme Encoded by ORF3 |
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Authors: | Michael Larsen Norio Gunge Friedhelm Meinhardt |
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Affiliation: | aInstitut für Mikrobiologie, Westfälische Wilhelms-Universität Münster, Corrensstrasse 3, 48149, Münster, Germany;bApplied Microbial Technology, Kumamoto Institute of Technology, 4-22-1 Ikeda, Kumamoto, 860, Japan |
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Abstract: | ORF3 of the cytoplasmic linear plasmid pGKL2 was disruptedin vivoby integration of a selectable marker. Long-term cultivation of transformants carrying hybrid plasmids with a disrupted ORF3 under selective pressure did not deprive strains of the native counterpart, thereby proving its essentiality for pGKL2 replication and maintenance. The predicted ORF3 polypeptide was found to contain conserved motifs acquainted with mRNA-capping enzymes in the required order, just as in cytoplasmic viruses; new conserved motifs were also identified. |
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Keywords: | killer linear plasmids mRNA capping guanylyltransferase cytoplasmic viruses |
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