Green Fluorescent Protein Purification by Organic Extraction |
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Authors: | Alexander V. Yakhnin Leonid M. Vinokurov Alexey K. Surin Yuli B. Alakhov |
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Affiliation: | aBranch of the Institute of Bioorganic Chemistry RAS, Pushchino, Moscow Region, 142292, Russia;bInstitute of Protein Research RAS, Pushchino, Moscow Region, 142292, Russia |
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Abstract: | Green fluorescent protein (GFP) is widely used as an excellent reporter molecule in biochemistry and cell biology. Some biochemical and immunological assays require high-purity GFP. However, the majority of current procedures for GFP purification include multiple time-consuming chromatography steps with a low yield of the desired product or require tag-containing proteins. An alternative method is described for the GFP purification without affinity extensions using organic extraction yielding a highly homogeneous protein indistinguishable in spectroscopic properties from that purified by previous methods. |
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