Expression, Purification, and Characterization of RecombinantEscherichia coliPyridoxine 5′-Phosphate Oxidase |
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| Authors: | Martino Di Salvo Emily Yang Genshi Zhao Malcolm E. Winkler Verne Schirch |
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| Affiliation: | aDepartment of Biochemistry and Molecular Biophysics, Institute for Structural Biology and Drug Discovery, Virginia Commonwealth University, Richmond, Virginia, 23298;bDepartment of Microbiology and Molecular Genetics, University of Texas Houston Medical School, Houston, Texas, 77030 |
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| Abstract: | A previously clonedpdxHgene fromEscherichia colicoding for pyridoxine 5′-phosphate oxidase was transferred to a pET22b vector and expressed inE. coliHMS174(DE3) cells. The soluble overexpressed enzyme was rapidly purified in high yield using two chromatography columns with an overall purification of about 2.8-fold. The purified enzyme contained tightly bound FMN. The enzyme exhibited the same spectral properties and similar kinetic constants to those previously reported by G. Zhao and M. E.Winkler (J. Bacteriol.177, 883, 1995), but differed from the properties reported by other investigators. A rapid procedure was developed for preparing apoPNP Ox in high yield. Both the holo- and apoenzymes were homodimers. The molar absorbtivity coefficient for the protein was determined for the fully active apoPNP Ox from is amino acid composition. Using this value and the spectral properties of the bound FMN it was shown by three different methods that the dimeric enzyme contains two molecules of bound FMN per dimer and not one FMN as previously reported. |
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