Conjugative Transfer of theEscherichia coli–Clostridium perfringensShuttle Vector pJIR1457 toClostridium botulinumType A Strains |
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| Authors: | Marite Bradshaw Michael C Goodnough Eric A Johnson |
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| Institution: | aDepartment of Food Microbiology and Toxicology, University of Wisconsin, Madison, Wisconsin, 53706;bDepartment of Bacteriology, University of Wisconsin, Madison, Wisconsin, 53706 |
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| Abstract: | An RP4-oriT shuttle vector pJIR1457 originally developed forClostridium perfringenswas successfully transferred by conjugation fromEscherichia colitoClostridium botulinumtype A strains and to a nontoxigenicC. botulinumtype A–transposon Tn916mutant strain lacking the entire toxin gene cluster. The light chain (LC) of botulinum toxin was highly expressed in the toxin deletion mutant strain from a pJIR1457 construct containing the recombinant botulinal gene for LC. This shuttle vector system will be valuable for genetic analysis ofC. botulinumand will enable genetic manipulation and recombinant expression studies of botulinum neurotoxins as pharmaceutical agents. |
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