Selective production of bikaverin in a fluidized bioreactor with immobilized Gibberella fujikuroi |
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Authors: | Escamilla-Silva Eleazar Poggi-Varaldo Héctor De la Torre-Martínez M. Mayra Sanchez Cornejo M.A. Guadalupe Dendooven Luc |
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Affiliation: | (1) Department of Biotechnology and Bioengineering, CINVESTAV – IPN, 14-740, CP 07000, Mexico D.F., Mexico |
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Abstract: | The best culture medium composition for the production of bikaverin by Gibberella fujikuroi in shake-flasks, i.e. 100 g glucose l–1; 1 g NH4Cl l–1; 2 g rice flour l–1; 5 g KH2PO4 l–1 and 2.5 g MgSO4 l–1, was obtained through a fractional factorial design and then scaled-up to a fluidized bioreactor. The effects of carbon and nitrogen concentrations, inoculum size, aeration, flow rate and bead sizes on batch bikaverin production using immobilized G. fujikuroi in a fluidized bioreactor were determined by an orthogonal experimental design. Concentrations of up to 6.83 g bikaverin l–1 were obtained when the medium contained 100 g glucose l–1 and 1 g NH4Cl l–1 with an inoculum ratio of 10% v/v, an aeration rate of 3 volumes of air per volume of medium min–1, and a bead size of 3 mm. Based on dry weight, the bikaverin production was 30–100 times larger than found in submerged culture and approximately three times larger than reported for solid substrate fermentation. |
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Keywords: | bikaverin fluidized bioreactor Gibberella fujikuroi immobilized orthogonal design |
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