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Expression of human apolipoprotein A-I in Nicotiana tabacum |
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| Authors: | Pasquale Chiaiese Maria Minutolo Angela Arciello Fulvio Guglielmini Renata Piccoli Edgardo Filippone |
| Affiliation: | (1) Department of Soil, Plant, Environmental and Animal Production Sciences, School of Biotechnological Sciences, University of Naples Federico II, Naples, Italy;(2) Department of Structural and Functional Biology, School of Biotechnological Sciences, University of Naples Federico II, Complesso Universitario di Monte S. Angelo Via Cinthia 4, 80126 Naples, Italy;(3) Istituto Nazionale di Biostrutture e Biosistemi (INBB), Rome, Italy |
| Abstract: | Several transgenic tobacco lines expressing human apolipoprotein A-I (ApoA-I) were obtained. Western blot analyses indicated the expression of the recombinant protein in plant organs at various stages of development, including senescent leaves. A cell line expressing human ApoA-I was established from a T1 transgenic plant. Recombinant ApoA-I was isolated either from extracts of transgenic leaves and from the culture medium of transgenic cells using an antibody-based one-step procedure. |
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