| 辽宁省番茄细菌性斑疹病的病原鉴定 |
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| 引用本文： | 苗则彦,李颖,赵杨. 辽宁省番茄细菌性斑疹病的病原鉴定[J]. 微生物学通报, 2013, 40(4): 603-608 |
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| 作者姓名： | 苗则彦 李颖 赵杨 |
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| 作者单位： | 辽宁省农业科学院 辽宁省农作物有害生物控制重点实验室 辽宁 沈阳 110161 |
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| 摘 要： | [目的]对在辽宁部分地区采集的番茄细菌性病害病原菌进行鉴定.[方法]对病原菌进行革兰氏染色、培养性状、生理生化特性、16S rRNA基因序列测定以及hrpZpst基因特异性扩增.[结果]鉴定该病原菌为丁香假单胞杆菌番茄致病变种[Pseudomonas syringae pv.tomato (Okabe) Young,Dye & Wilkie].[结论]利用hrpZpst基因特异性扩增可以快速鉴定病原菌.
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| 关 键 词： | 番茄细菌性斑疹病 丁香假单胞杆菌番茄致病变种 hrpZpst基因 |
Pathogen identification of bacterial speck of tomato in Liaoning |
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| MIAO Ze-Yan,LI Ying and ZHAO Yang. Pathogen identification of bacterial speck of tomato in Liaoning[J]. Microbiology China, 2013, 40(4): 603-608 |
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| Authors: | MIAO Ze-Yan LI Ying ZHAO Yang |
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| Affiliation: | Liaoning Academy of Agricultural Sciences, Liaoning Key Laboratory of Crop Pest Management, Shenyang, Liaoning 110161, China |
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| Abstract: | [Objective] Identification of bacterial disease of tomato in Liaoning. [Methods] According to result of the pathogenicity, physiological and biochemical characterizations, 16S rDNA sequence and specific amplification of hrpZpst gene. [Results] The isolates were identified as Pseudomonas syringae pv. tomato (Okabe). [Conclusion] hrpZpst gene can be used to identify Pseudomonas syringae pv. tomato (Okabe). |
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| Keywords: | Bacterial speck of tomato Pseudomonas syringae pv. tomato hrpZpst gene |
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