A DNA vaccine candidate for <Emphasis Type="Italic">B. anthracis</Emphasis> immunization,pcDNA3.1+PA plasmid,induce Th1/Th2 mixed responses and protection in mice |
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Authors: | Fatemeh Vahedi Jennelle Kyd Gholamreza Moazeni Jula Monavvar Afzalaghaeei Mahdi Kianizadeh Mahmoud Mahmoudi |
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Institution: | (1) Biotechnology Department, Razi Vaccine & Serum Research Institute, Mashhad, Iran;(2) Capricornia Center of Mucosal Immunology, Central Queensland University, Rock Hampton, Australia;(3) Department of Aerobic Bacterial Vaccine Production, Razi Vaccine & Serum Research Institute, Karaj, Iran;(4) Department of Social Medicine, Mashhad University of Medical Science, Mashhad, Iran;(5) Immunology Research Center, Mashhad University of Medical Science, Mashhad, Iran |
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Abstract: | The protective antigen (PA) of Bacillus anthracis (B. anthracis) is a potent immunogen and a candidate subunit vaccine. To address the question whether antibodies raised against PA following
injection of pcDNA3.1+PA plasmid, encoding PA, can protect against virulent B. anthracis two different regimens of PA based vaccines (DNA and live spore) were used. The groups of BALB/c mice that received live
spores of the Sterne strain, naked pcDNA3.1 and naked pcDNA3.1+PA were compared to control groups. All groups were injected
three times with 30-day intervals. Two weeks after the last immunization, all mice were subjected to challenge with a pathogenic
strain of B.
anthracis (C2). Blood samples were taken before each injection and challenge. Evaluation of the sera by ELISA method showed that DNA
immunization using pcDNA3.1+PA plasmid resulted in an antibody profile representative of a mixed Th1 and Th2 response, with
a skewing to a Th1 response. The group which received the naked pcDNA3.1+PA had a survival rate of >80%. This challenge assay
revealed that antibodies raised following DNA vaccination against PA can confer strong protection, and resistance against
virulent species of B. anthracis. |
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Keywords: | DNA vaccine Bacillus anthracis pcDNA3 1 |
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