Effect of tunicamycin on α-galactosidase secretion by Aspergillus nidulans and the importance of N-glycosylation |
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Authors: | Santiago Rí os,Immaculada Ferná ndez-Monistrol,Fernando Laborda |
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Affiliation: | Departamento de Microbiología y Parasitología, Facultad de Farmacia, Universidad de Alcaláde Henares, Campus Universitario, 28871 Alcaláde Henares, Madrid, Spain |
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Abstract: | Abstract Aspergillus nidulans released α-galactosidase into the culture medium during the exponential growth on either lactose or galactose as the only carbon source. This enzyme is a glycoprotein. Its treatment with endoglycosidases produces a reduction in its molecular mass but the resulting enzyme conserved some of their carbohydrate components in addition to its enzymatic activity. Mycelia of A. nidulans growing in the presence of tunicamycin synthesized an underglycosylated α-galactosidase which was not released into the culture media but remained bound to the cell-wall. Tunicamycin did not prevent the synthesis and secretion of α-galactosidase by protoplasts. N-linked oligosaccharide chains seem not to be essential for the synthesis and secretion of α-galactosidase of A. nidulans , but they could be necessary for proper targeting at the extracellular level. |
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Keywords: | α-galactosidase Aspergillus nidulans Secretion |
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