Essential role of phosphatidylinositol 3-kinase in insulin-induced activation and phosphorylation of the cGMP-inhibited cAMP phosphodiesterase in rat adipocytes studies using the selective inhibitor wortmannin |
| |
Authors: | Tova Rahn, Martin Ridderstr le, Hans Tornqvist, Vincent Manganiello, Gudrun Fredrikson, Per Belfrage,Eva Degerman |
| |
Affiliation: | Tova Rahn, Martin Ridderstråle, Hans Tornqvist, Vincent Manganiello, Gudrun Fredrikson, Per Belfrage,Eva Degerman |
| |
Abstract: | Incubation of rat adipocytes with wortmannin, a potent and selective phosphatidylinositol 3-kinase (PI 3-kinase) inhibitor, completely blocked the antilipolytic action of insulin (IC50≈ 100 nM), the insulin-induced activation and phosphorylation of cGMP-inhibited cAMP phosphodiesterase (cGI-PDE) as well as the activation of the insulin-stimulated cGI-PDE kinase (IC50≈ 10–30 nM). No direct effects of the inhibitor on the insulin-stimulated cGI-PDE kinase, the cGI-PDE and the hormone-sensitive lipase were observed. These data suggest that activation of PI 3-kinase upstream of the insulin-stimulated cGI-PDE kinase in the antilipolytic insulin signalchain has an essential role for insulin-induced cGI-PDE activation/ phosphorylation and anti-lipolysis. |
| |
Keywords: | Insulin Phosphatidylinositol 3-kinase Inhibitor Adipocyte Lipolysis cGMP-inhibited cAMP phosphodiesterase |
本文献已被 ScienceDirect 等数据库收录! |
|