Removal of 2,4-dinitrotoluene from concrete using bioremediation,agar extraction,and photocatalysis |
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Authors: | S. R. Phutane J. N. Renner S. L. Nelson W. S. Seames J. Páca T. J. Sundstrom E. I. Kozliak |
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Affiliation: | Department of Chemical Engineering, University of North Dakota, Grand Forks, ND 58202, USA. |
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Abstract: | Three methods, i.e. bioremediation by application of bacteria-laden agar, physical absorption of DNT by agar, or illumination by UV light were evaluated for the removal of 2,4-dinitrotoluene (DNT) from building-grade concrete. DNT biodegradation by Pseudomonas putida TOD was turned "on" and "off" by using toluene as a co-substrate thus allowing for rate-limiting step assessment. Bioremediation efficiency can be > 95-97% in 5-7 d if the process occurs at optimum growth temperature with the biological processes appearing to be rate-limiting. Sterile agar can remove up to 80% of DNT from concrete thus allowing DNT desorption and biodegradation to be conducted separately. Photoremediation results in 50% DNT removal in 9-12 d with no further removal, most likely due to mass transfer limitations. |
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